Myc shrna
WebFigure 1 The expression of MYC or PLK1, and basal autophagy level in osteosarcoma cells. (A and B) Total RNA were prepared, and subjected to qPCR.ACTB was used as an internal reference. (C and D) Whole cell lysates were prepared, and subjected to Western blot analysis using indicated antibodies.ACTB was used as a loading control. Treatment of 5 … WebIn HCT-116 and HT-29 cell, we adopted shRNA to silence c-myc expression and examined the effects. As shown in Figure 4A – B , in c-Myc knockdown cells, the expression of hexokinase-2 was dramatically decreased, the glucose consumption and lactate production were significantly dropped accordingly.
Myc shrna
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Web4 feb. 2024 · Two shRNA against ACTL6A and one shRNA against MYC in pSuper-retro-neo vector were purchased from Sigma-Aldrich. Transfection of plasmids was performed using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, California, USA) according to the manufacturer’s instructions. Web19 feb. 2024 · Specifically, overexpression of TGFβ1 hampers hepatocarcinogenesis driven by c-Myc. Consistently, shRNA mediated silencing of Smad2, Smad3, or Smad4, or overexpression of inhibitory Smad7 (data ...
Web3 sep. 2024 · Graphical Abstract. A multisite-targeting c-Myc shRNA array-based transcription nanovector was efficiently constructed through branch-PCR, which greatly … Web13 apr. 2024 · Silencing TMEM40 with shRNA inhibited the invasion, migration and proliferation, promoted apoptosis and led to a G0/G1 cell cycle arrest in CC cell lines. …
WebCont, control. (f) Effects of c-Myc shRNA on the Axl promoter activity by ADI. The cells were transfected with c-Myc shRNA and its control scramble shRNA for 48 h. Cells were treated with ADI for 6 h. Web14 jun. 2024 · This study explores the effects of C-myc gene silencing on cell proliferation, apoptosis and cytokine expression in interleukin (IL)-1β-induced rat chondrocytes. Primary chondrocytes were obtained from 40 Sprague-Dawley rats. For in vitro C-myc3-shRNA transfection, chondrocytes were assigned to a blank 1, model 1, IL-1β + C-myc3-shRNA, …
Web12 jun. 2024 · The sEVs of Myc-manipulated SCLC cells were isolated using total exosome isolation reagents (from cells) (Life Technologies, USA). The sEVs were harvested from …
WebFigure 1 The expression of MYC or PLK1, and basal autophagy level in osteosarcoma cells. (A and B) Total RNA were prepared, and subjected to qPCR.ACTB was used as an … pokemon x reflection cave walkthroughWeb11 apr. 2024 · STAT3 inhibition attenuates MYC expression by affecting recruitment of histone acetyltransferase p300, thereby reducing enrichment of H3K27 acetylation in the MYC promoter. Concomitantly, it also decreases the occupancy of the bromodomain containing ... using inducible STAT3 shRNA and a clinically relevant STAT3 inhibitor … pokemon x reflection cave mapWeb20 sep. 2013 · YAP or c-Myc-specific shRNAs with high knockdown efficiency ( Fig. 1A and B) were used to silence protein expression in both Bel-7402 and SMMC-7721 cells. We found that inhibition of either YAP or c-Myc decreased cell proliferation compared to control, as measured by a MTT-based assay (Fig. 1 C). pokemon x mystery gift codesWebUpon transient transfection with plasmid-encoding shRNA, it was found that expression of c-Myc decreased in shRNA-transfected cells, and the downregulation of c-Myc inhibited … pokemon x rom download romsmaniaWebFigure 1 Knockdown of PCGF1 by lentivirus-mediated short hairpin RNA (shRNA)-induced cell cycle arrested at G0/G1 phase and attenuation of proliferation in U87 cells.Notes: (A) Expression analysis of PCGF1 mRNA by RT-qPCR analysis.(B) Expression of PCGF1 proteins in infected cells was analyzed by Western blot.(C) Cell cycle distribution of U87 … pokemon x on computerWebSince the differential protein expression of MYC is a regulator of the RU/RR dichotomy and is higher in RR cells, we asked if MYC regulates the autophagy-mediated cytoprotective effect. Inhibition of MYC in RR cells using shRNA significantly blunted crizotinib-induced autophagic response and effectively suppressed this cytoprotective effect. pokemon x shiny stone locationWebshRNA sequences correspond to N-Myc siRNA Gene Silencer sequences; provided as transfection-ready purified plasmid DNA target-specific gene silencing; after transfection, … pokemon x shiny cheat code