Cisplatin hplc method

WebMar 1, 2010 · Cisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human … WebAug 15, 2007 · The HPLC method was developed for individual as well as simultaneous determination of two drugs namely carboplatin and paclitaxel in the presence of polymer …

RP-HPLC METHOD FOR THE QUANTIFICATION OF …

WebThe method was successfully applied for routine analysis of Oxaliplatin tablet dosage form and bulk drug. Key Words:Oxaliplatin, RP-HPLC, UV detection, recovery, precise, 240nm INTRODUCTION Oxaliplatin is a platinum-based antineoplastic agent that is used in cancer chemotherapy. oxaliplatin is the platinum-containing complex similar to cisplatin. WebMaterials and Methods Chemicals. Reduced glutathione (GSH), glutathione oxidized, and g-glutamyl glutamate, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, solvents of analytical grade (methanol and glacial acetic acid) used for HPLC, thioctic acid (a-lipoic acid, oxidized form) and cisplatin were purchased ... polymem silver non-adhesive pad dressing https://superior-scaffolding-services.com

In vivo detection of DNA adducts induced by cisplatin using ... - PubMed

WebMar 11, 2024 · Cisplatin (CDDP), a platinum-based drug, is the most potent anti-cancer agent and is widely used for cancer treatment [ 1 ]. In particular, it plays crucial roles in … WebAug 23, 2012 · Establishing HPLC/ICP-MS quantification methods. For quantification of Cisplatin–DNA adducts, IDA was chosen. For the adduct d(GpG)-Pt(NH 3) 2, Garcia Sar et al. already published a suitable approach. 21 In order to monitor multiple adducts, we have chosen species-unspecific IDA (SU-IDA). With SU-IDA it is possible to study the … WebDec 1, 2024 · Tezcan et al. [70] described a HPLC-PDA method for monitoring the plasma concentration of free cisplatin in different types of cancer. Deproteinization with cold MeOH was used for sample extraction of cisplatin and NiCI 2 .6.H 2 O as IS. shani wallis and julie christie

HPLC methods for quantifying anticancer drugs in human …

Category:Development of a novel chromatographic method for concurrent ...

Tags:Cisplatin hplc method

Cisplatin hplc method

Determination of Liposomal Cisplatin by High-Performance Liquid Chro…

WebCisplatin was the first of the platinum-based chemotherapeutic agents and therefore has been extensively studied as an antitumor agent since the late 1960s. Because this agent … http://www.ijsit.com/admin/ijsit_files/ijsit_1.1.5.pdf

Cisplatin hplc method

Did you know?

WebSep 1, 2024 · Application of a newly developed HPLC method to evaluate the drug release of co-loaded 5-fluorouracil and cisplatin films. Abstract The combination of 5-fluorouracil (5-FU) and cisplatin (Cis) has been used for the treatment of many cancers. WebJun 13, 2005 · Cisplatin, cisplatinum or cis-diamminedichloroplatinum (II) (CDDP) is a platinum-based chemotherapy drug used to treat various types of cancers, including sarcomas, some carcinomas (e.g. small cell lung cancer, and ovarian cancer), lymphomas and germ cell tumors. It was the first member of its class, which now also includes …

WebFor cis-diammine (1,1-cyclobutane-dicarboxylato) platinum (CBDCA), normal-phase chromatography on silica with UV detection suffices down to levels of 10 μM in urine or plasma ultrafiltrates. Quantitation below 10 μM needs HPLC fraction collection followed by flameless atomic absorption spectrophotometry (FAAS; 0.5 μM detectable). Webinjected onto the HPLC system. Retention time of cisplatin, mono-chloride, OH-dimer, and none-chloride was 3.2, 3.4, 3.6, and, 4.3–6.6 min, respectively. Measurable ranges with this method were 1˜10˚5 to 4˜10˚3 M for cisplatin. Correlation coefficient of the calibration curves of cisplatin was 0.999 (p<0.01). The within- and

WebHigh-performance liquid chromatography of cisplatin The retention behavior of cisplatin on a variety of stationary phases has been investigated using aqueous mobile phases … WebA method of preparation and physical properties including the pK a of several species of cisplatin in water,2 the UV and visible spectra, HPLC methods, and mass spectra have been reported.1 Cisplatin is a platinum-containing, broad activity antineoplastic and alkylating agent effective against solid tumors of testes, ovaries, and bladder ...

WebApr 1, 2012 · The HPLC method was developed using C 18 ODS Hypersil column of 250 × 4.6 mm id with 5 µm particle size, mobile phase of water : methanol : acetonitrile (40:35:25, v/v/v, pH 3.5) at a flow rate...

WebSep 1, 2016 · An isocratic reversed-phase high-performance liquid chromatographic method with ultraviolet detection at 254 nm has been developed for the determination of doxorubicin in human plasma. Plasma... shani vedic mantrahttp://www.ijsit.com/admin/ijsit_files/ijsit_1.1.5.pdf shani wallis actressWebApr 24, 2024 · Doxorubicin hydrochloride. Several methods such as LC-MS, reverse phase HPLC and UV-Visible spectrometry have been reported and validated for quantitative analysis of doxorubicin hydrochloride in bulk and pharmaceutical dosage forms [12,13,14, 22, 23].All these methods require use of solvents, however due to unstable … shani wallis oliverWebJun 12, 2000 · In water, the antineoplastic drug cisplatin, cis-[PtCl2(NH3)2] (1) hydrolyses slowly to the aqua complexes cis-[Pt(NH 3) 2 Cl(H 2 O)] + (2) and, to a small extent, cis-[Pt(NH 3) 2 (H 2 O) 2] 2+ (3), which are thought to play an important role in the metabolism of cisplatin.HPLC is a useful technique for monitoring 2 and 3, but only if the … shani wallis picsWebThe HPLC system (LC-20AD model, Shimadzu, Kyoto, Japan) consisted of two pumps (LC 20-AT model), an automatic injector (9SIL-20AD model), an oven (CTO-20AS model), a spectrophotometric detector (SPD-M20A model), and a computer equipped with the analysis software Shimadzu LC. shani wallace actressWebderivitized cisplatin [21]. The objective of study was to develop and validate a direct and effective HPLC method for the simultaneous determination of etoposide and cisplatin which could be used in quality control analysis. The present work therefore is a direct method with good linearity and sensitivity for both drugs. O O O H HO H HO O O O O ... shani wallis biographyWebTotal and free cisplatin concentrations were measured using a validated HPLC-UV method. Results: The highest concentration of total and free cisplatin was achieved at the end of the infusion in both regimens. Total cisplatin concentration declined 30 minutes after the end of infusion in both the groups. After 1hour of discontinuing cisplatin ... shani vedic beej mantra